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anti gap 43  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti gap 43
    Anti Gap 43, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+gap+43/GAP43+Rabbit+mAb/pm41566018-143-27-29
    Average 93 stars, based on 93 article reviews
    anti gap 43 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Small extracellular vesicles derived from mesenchymal stem cell facilitate functional recovery in spinal cord injury by activating neural stem cells via the ERK1/2 pathway
    Article Snippet: Immunohistochemical staining was performed according to the manufacturer’s protocols (Boster, Wuhan, China). .. To the stain spinal cord tissues, sections were incubated with various primary antibodies at 4°C overnight, including anti-Gap-43(1:100, CST, United States), anti-NF200 (Boster, China), anti-SYP (1:100, Abcam, United States), and anti-PSD95 (Boster, China). ..

    Article Title: 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.
    Article Snippet: .. Immunofluorescence Staining and Confocal Imaging Configuration: Primary antibodies were anti-Beta-Tubulin (ab6046, Abcam, UK, 1:200 dilution), anti-GAP-43 (8945, Cell Signaling Company, USA, 1:200 dilution), and anti-EGFR (M3563, DAKO, Germany, 1:500 dilution, or ab76153, Abcam, UK, 1:200 dilution). .. Secondary antibodies were Alexa Flour 488 anti-rabbit (A11008, Life Technologies, USA, 1:500 dilution) and Alexa Fluor 647 anti-mouse (A21240, Life Technologies, USA, 1:500 dilution).

    Article Title: Agent for use in the treatment of glioma
    Article Snippet: .. The following antibodies were used for 10 μm cryotome sections and ICCs: anti-nestin (ab6320, Abcam, specific staining of the human nestin intermediate filament, which is highly expressed in GBMSCs, but not detectable in normal mouse brain), in combination with anti-β-catenin (ab16051, Abcam), anti-β-parvin (sc-50775, Santa Cruz), anti-beta tubulin III (ab18207, Abcam), anti-Cx26 (ab59020, Abcam), anti-Cx31 (ab156582, Abcam), anti-Cx37 (ab185820, Abcam), anti-Cx43 (C6219, Sigma), anti-GAP-43 (8945, Cell Signaling), anti-GFAP (Z0334, Dako), anti-Ki-67 (M7240, Dako), anti-myosin IIa (ab24762, Abcam), anti-myosin X (22430002, Novus Bioscience), anti-N-Cadherin (ab18203, Abcam), and anti-PDI (ab3672, Abcam). .. Images were acquired using a Leica SP5 confocal microscope.

    Incubation:

    Article Title: Small extracellular vesicles derived from mesenchymal stem cell facilitate functional recovery in spinal cord injury by activating neural stem cells via the ERK1/2 pathway
    Article Snippet: Immunohistochemical staining was performed according to the manufacturer’s protocols (Boster, Wuhan, China). .. To the stain spinal cord tissues, sections were incubated with various primary antibodies at 4°C overnight, including anti-Gap-43(1:100, CST, United States), anti-NF200 (Boster, China), anti-SYP (1:100, Abcam, United States), and anti-PSD95 (Boster, China). ..

    Article Title: Agent for use in the treatment of glioma
    Article Snippet: .. Human sections were incubated with anti-IDH1 R132H (H09, Dianova), anti-Cx43 (C 6219, Sigma), anti-GAP-43 (8945, Cell Signaling), anti-NGF (ab52918, Abcam), anti-NT4 (ab150437, Abcam), anti-TrkA (ab76291, Abcam) and anti-TrkB (ab134155, Abcam) antibodies. ..

    Article Title: The therapeutic potential and mechanisms of targeting the PTBP1/Nogo-A/NgR axis in PTSD induced by single prolonged stress in mice
    Article Snippet: Subsequently, the proteins were transferred to polyvinylidene fluoride membranes (0.2 μm, Millipore, cat. no. ISEQ00010; or 0.45 μm, Millipore, cat. no. IPVH00010). .. The membranes were blocked with 5% nonfat milk at room temperature for 1 h. Primary antibodies, including anti-PTBP1 (1:2,000, Abcam, ab133734), anti-RTN4R (1:7,000, Boster, A02250-2), anti-NeuN (1:1,000, Millipore, MAB377), anti-Bcl-2 (1:1,000, Sigma, SAB450003), anti-Bax (1:1,000, CST, 14796S), anti-Caspase-3 and anti-Cleaved Caspase-3 (1:1,000, Abcam, ab13847), anti-PSD95 (1:7,000, CST, 36233S), anti-SYN1 (1:7,000, Boster, BA1421), anti-NF200 (1:1,000, Abcam, ab8135), anti-GAP-43 (1:1,000, CST, 8945S), anti-Nogo-A (1:1,000, Abcam, ab47085), anti-RhoA (1:1,000, CST, 2117S), anti-ROCK2 (1:1,000, CST, 9029S), anti-β-actin (1:7,000, Sigma, A1978) and anti-GAPDH (1:7,000, CST, 5174S), were incubated with the membranes overnight at 4 °C. ..

    Blocking Assay:

    Article Title: Agent for use in the treatment of glioma
    Article Snippet: .. After blotting and blocking, the following antibodies were used over night at 4° C.: anti-Cx26 (ab59020, Abcam), anti-Cx31 (ab156582, Abcam), anti-Cx37 (ab185820, Abcam), anti-Cx43 (C 6219, Sigma), anti-GAP-43 (8945, Cell Signaling). .. As loading control, anti-GAPDH antibody (C4780, Linaris), or anti-alpha-tubulin antibody (T9026, Sigma) was used.

    Immunofluorescence:

    Article Title: 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.
    Article Snippet: .. Immunofluorescence Staining and Confocal Imaging Configuration: Primary antibodies were anti-Beta-Tubulin (ab6046, Abcam, UK, 1:200 dilution), anti-GAP-43 (8945, Cell Signaling Company, USA, 1:200 dilution), and anti-EGFR (M3563, DAKO, Germany, 1:500 dilution, or ab76153, Abcam, UK, 1:200 dilution). .. Secondary antibodies were Alexa Flour 488 anti-rabbit (A11008, Life Technologies, USA, 1:500 dilution) and Alexa Fluor 647 anti-mouse (A21240, Life Technologies, USA, 1:500 dilution).

    Imaging:

    Article Title: 3D-Engineered Scaffolds to Study Microtubes and Localization of Epidermal Growth Factor Receptor in Patient-Derived Glioma Cells.
    Article Snippet: .. Immunofluorescence Staining and Confocal Imaging Configuration: Primary antibodies were anti-Beta-Tubulin (ab6046, Abcam, UK, 1:200 dilution), anti-GAP-43 (8945, Cell Signaling Company, USA, 1:200 dilution), and anti-EGFR (M3563, DAKO, Germany, 1:500 dilution, or ab76153, Abcam, UK, 1:200 dilution). .. Secondary antibodies were Alexa Flour 488 anti-rabbit (A11008, Life Technologies, USA, 1:500 dilution) and Alexa Fluor 647 anti-mouse (A21240, Life Technologies, USA, 1:500 dilution).



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